Vitrification of mouse oocytes using closed pulled straws (CPS) achieves a high survival and preserves good patterns of meiotic spindles, compared with conventional straws, open pulled straws (OPS) and grids
نویسندگان
چکیده
منابع مشابه
Open pulled straws for vitrification of mature mouse oocytes preserve patterns of meiotic spindles and chromosomes better than conventional straws.
Vitrification of oocytes has been applied recently for humans, but remains elusive. The microtubules of oocytes are vulnerable to cryoprotectants and thermal changes. Using mouse oocytes, the effects of vitrification in open pulled straws (OPS) were investigated on survival, the meiotic spindle, and chromosomes and compared with conventional straws. Mature oocytes were allocated to four groups ...
متن کاملAseptic technology of vitrification of human pronuclear oocytes using open-pulled straws.
BACKGROUND The aim of this study was to compare the viability of human pronuclear oocytes subjected to vitrification using cooling by direct submerging of open-pulled straws in liquid nitrogen versus vitrification by cooling of open-pulled straws located inside a closed 0.5 ml straw (aseptic system). METHODS Two- and three-pronuclei stage oocytes (n=114) were cryopreserved in super-open-pulle...
متن کاملThe effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivo produced bovine embryos
The aim of this study was to compare pregnancy rates after transfer of in vivo produced embryos cryopreserved using open pulled straw (OPS) vitrification (Group V) or conventional freezing method as a control (Group C). Bovine embryos (Day6.5–7.5) collected from superovulated cows were classified according to developmental stages and morphological qualities (Grade 1 and 2) before cryopreservati...
متن کاملComparison of The Effects of Vitrification on Gene Expression of Mature Mouse Oocytes Using Cryotop and Open Pulled Straw
Objective Oocyte cryopreservation is an essential part of assisted reproductive technology (ART) which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems (Cryotop and Open Pulled Straw, OPS) on mature oocytes gene expressions. MaterialsAndMethods The survival rate of metaphase II (MII) mouse oocytes were assessed after cryoprese...
متن کاملEffect of vitrification on number of inner cell mass in mouse blastocysts in conventional straw, closed pulled straw, open pulled straw and cryoloop carriers.
OBJECTIVE To compare the effect of using open and closed carriers on count of inner cell mass in vitrified mouse blastocyst after warming. METHODS The experimental study was conducted at Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, from April to September 2010. Forty female NMRI (Naval Medical Research Institute, USA) mice were injected with pregnant mare's serum gonadotropi...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2001
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/16.11.2350